What are the molecular features of the different cholangiocarcinoma (CCA) subtypes?

CCA can be anatomically divided into three different subtypes: distal, perihilar, and intrahepatic. Distal and perihilar originate from the common bile duct, whereas intrahepatic originates from the intrahepatic bile duct cells. While these anatomical differences are understood, our lab is interested in the molecular features of each type of CCA, which have yet to be fully elucidated. We have developed an in vitro model using organoids derived from intrahepatic and common bile duct cells from mice harboring human CCA oncogenic mutations.